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rat trpm8 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc rat trpm8 plasmid
    Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and <t>TRPM8</t> channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.
    Rat Trpm8 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat trpm8 plasmid/product/Addgene inc
    Average 90 stars, based on 6 article reviews
    rat trpm8 plasmid - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Identification of Phialomustin‑B (PHL-B) as a Novel Natural TRPA1 Channel Inhibitor"

    Article Title: Identification of Phialomustin‑B (PHL-B) as a Novel Natural TRPA1 Channel Inhibitor

    Journal: ACS Omega

    doi: 10.1021/acsomega.5c06063

    Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and TRPM8 channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.
    Figure Legend Snippet: Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and TRPM8 channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.

    Techniques Used: Concentration Assay, Fluorescence



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    rat trpm8 plasmid  (Addgene inc)
    90
    Addgene inc rat trpm8 plasmid
    Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and <t>TRPM8</t> channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.
    Rat Trpm8 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat trpm8 plasmid/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    rat trpm8 plasmid - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    pegfp trpm8 rat  (Addgene inc)
    90
    Addgene inc pegfp trpm8 rat
    Time course of NGF-induced changes in fluorescence intensity. NGF (100 ng/mL) was applied during the times indicated by the black bar/gray shading. Traces represent the mean, error bars are SEM. Control and TRPV1 data same as in with error bars removed for clarity. (A-B) Averaged normalized TIRF intensity of Akt-PH from cells transfected with TrkA/p75 NTR and Akt-PH and: (A) no channel (control; blue; n=75); TRPV1 (orange; n=122); TRPV2 (black; n=61); TRPV4 (yellow; n=29) (B) no channel (control; blue; n=75); TRPM4 (n=63); <t>TRPM8</t> (n=73); TRPA1 (n=59). (C-D) Averaged normalized TIRF intensity of individual TRP channels. Color scheme as in (A-B) with the cell numbers as follows: (C) TRPV1 (n=94); TRPV2 (n=62); TRPV4 (n=48); (D) TRPM4 (n=65); TRPM8 (n=67); TRPA1 (n=52).
    Pegfp Trpm8 Rat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pegfp trpm8 rat/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pegfp trpm8 rat - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and TRPM8 channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.

    Journal: ACS Omega

    Article Title: Identification of Phialomustin‑B (PHL-B) as a Novel Natural TRPA1 Channel Inhibitor

    doi: 10.1021/acsomega.5c06063

    Figure Lengend Snippet: Lack of inhibitory effect of PHL-B on TRPV1, TRPV4, and TRPM8 channels. (A, C, E) Concentration-dependent relationships of PHL-B on TRPV1, TRPV4, and TRPM8 channels upon stimulation with capsaicin, HTS, and menthol, respectively. Capsazepine, HC-067047, and sesamin were used as reference inhibitors for TRPV1, TRPV4, and TRPM8 channels, respectively. (B, D, F) Plots of normalized fluorescence vs drug concentration at different time points for each channel, where the lack of effect of PHL-B can be clearly visualized. These data could not be fitted with a Hill equation; hence, the IC 50 values were not determined. Data are expressed as means ± SEM, N = 3–5 independent experiments.

    Article Snippet: The rat TRPM8 plasmid was obtained from Addgene (#64879).

    Techniques: Concentration Assay, Fluorescence

    Time course of NGF-induced changes in fluorescence intensity. NGF (100 ng/mL) was applied during the times indicated by the black bar/gray shading. Traces represent the mean, error bars are SEM. Control and TRPV1 data same as in with error bars removed for clarity. (A-B) Averaged normalized TIRF intensity of Akt-PH from cells transfected with TrkA/p75 NTR and Akt-PH and: (A) no channel (control; blue; n=75); TRPV1 (orange; n=122); TRPV2 (black; n=61); TRPV4 (yellow; n=29) (B) no channel (control; blue; n=75); TRPM4 (n=63); TRPM8 (n=73); TRPA1 (n=59). (C-D) Averaged normalized TIRF intensity of individual TRP channels. Color scheme as in (A-B) with the cell numbers as follows: (C) TRPV1 (n=94); TRPV2 (n=62); TRPV4 (n=48); (D) TRPM4 (n=65); TRPM8 (n=67); TRPA1 (n=52).

    Journal: bioRxiv

    Article Title: Reciprocal regulation among TRPV1 channels and phosphoi nos itide 3-kinase in response to nerve growth factor

    doi: 10.1101/346718

    Figure Lengend Snippet: Time course of NGF-induced changes in fluorescence intensity. NGF (100 ng/mL) was applied during the times indicated by the black bar/gray shading. Traces represent the mean, error bars are SEM. Control and TRPV1 data same as in with error bars removed for clarity. (A-B) Averaged normalized TIRF intensity of Akt-PH from cells transfected with TrkA/p75 NTR and Akt-PH and: (A) no channel (control; blue; n=75); TRPV1 (orange; n=122); TRPV2 (black; n=61); TRPV4 (yellow; n=29) (B) no channel (control; blue; n=75); TRPM4 (n=63); TRPM8 (n=73); TRPA1 (n=59). (C-D) Averaged normalized TIRF intensity of individual TRP channels. Color scheme as in (A-B) with the cell numbers as follows: (C) TRPV1 (n=94); TRPV2 (n=62); TRPV4 (n=48); (D) TRPM4 (n=65); TRPM8 (n=67); TRPA1 (n=52).

    Article Snippet: TRPV4-EGFP (human) in pEGFP was obtained from Tim Plant (Charite-Universitatsmedizine, Berlin) ( ). pEGFP-TRPM8 (rat) in pEGFP was obtained from Addgene (#64879, Addgene, Cambridge, MA).

    Techniques: Fluorescence, Transfection